ABSTRACT
Objective To investigate the role of Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3) signaling pathway in the pathogenesis of type 2 diabetic macroangiopathy. Methods The human umbilical vein endothelial cells (HUVECs) were incubated for 24 h with the serum of healthy volunteers, simple type 2 diabetic patients and patients with type 2 diabetic macroangiopathy. JAK2 specific inhibitor AG490 was used to block the JAK2/STAT3 signaling pathway. According to different treatments, the cells were divided into normal control group (NC group, n=30), simple diabetes mellitus group (DM group, n=30), type 2 diabetic macroangiopathy group (DV group, n=30), DM+AG490 group (DM+AG490 group, n=30) and DV+AG490 group (DV+AG490 group, n=30). Real-time quantitative PCR technique was used to detect the mRNA expression of JAK2, STAT3, vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (FLT1) in each group. Western blotting analysis was used to detect the protein expression of JAK2, STAT3 and phosphorylated STAT3(p-STAT3). Results Compared with the NC group, the expression of JAK2, STAT3 mRNA and JAK2, and p-STAT3 protein were significantly up-regulated in DM and DV groups (P<0.05), and the expression of JAK2, STAT3 mRNA and JAK2, p-STAT3 protein in DV groups were significantly higher than those in DM group (P< 0.05). The expression of JAK2, STAT3 mRNA and JAK2, p-STAT3 protein in DM+AG490 group and DV+AG490 group were significantly lower than those in the DM group and DV group (P< 0.05). Compared with the NC and DM group, the expression of VEGF, FLT1 mRNA was significantly up-regulated in DV group(P<0.05). Compared with the DV group, the expression of VEGF and FLT1mRNA were significantly reduced in DV+AG490 group (P< 0.05). Conclusion JAK2/STAT3 signaling pathway may play a role in the pathogenesis of type 2 diabetic macroangiopathy.
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<p><b>OBJECTIVE</b>To construct a MYH9 gene knockout model in MGC803 cell line using transcription activator-like effector nuclease (TALEN) and observe its effect on cell cycle and apoptosis.</p><p><b>METHODS</b>According to FastTALE(TM) TALEN Kit, we designed TALEN pairs and constructed the plasmids targeting to MYH9 gene. After detecting their activity in MGC803 cells by plasmid transfection, DNA sequencing, RT-PCR and western blot, we selected the monoclonal cells and studied the changes in the cell cycle and apoptosis.</p><p><b>RESULTS</b>MYH9 gene could not be knocked out but knocked down in selected MGC803 monoclonal cells, which caused cell cycle arrested at G2/M phase (P<0.05) and a significant increase in the cell number with early apoptosis (P<0.01).</p><p><b>CONCLUSION</b>We successfully generated a MYH9 knockdown model in MGC803 cell lines by TALEN, which could be in favor of MYH9 function study in gastric cancer.</p>
Subject(s)
Humans , Apoptosis , Cell Cycle , Cell Line, Tumor , Cell Proliferation , Gene Knockdown Techniques , Molecular Motor Proteins , Genetics , Myosin Heavy Chains , Genetics , Plasmids , Stomach Neoplasms , TransfectionABSTRACT
Objective To examine the composition and distribution of animal reservoir of plague in Quanzhou city, Fujian province, and so as to accumulate basic data for making the plague prevention and control measures. Methods Rodents were captured by cages in six monitoring spots of Quanzhou city between 2000 and 2009. Then rodents and flea species were identified. Rats liver and spleen homogenates were detected of plague F1 antigen by reverse indirect hemagglutination test (RIHA); rats serum were detected of plague F1 antibody by indirect hemagglutination test (IHA). ResultsA total of 26 264 rodents were captured between 2000 and 2009.Rattus norvegicus and Rattus flavipectus were dominant species, which were accounted for 45.97％ (12 074/26 264)and 32.01％(8407/26 264), respectively. The account of Rattus flavipectus captured between 2005 and 2009 was (26.99 ± 2.46)％ and (37.03 ± 3.79)％ between 2000 and 2004. The difference was statistically significant (t =4.97, P ＜ 0.05). Total rodent densities was (6.86 ± 1.44)％, including(5.36 ± 1.83)％ in mountains and (6.81 ±1.66)％ in coastal areas, respectively, and the difference was not statistically significant(t =1.01, P ＞ 0.05). Total flea index and flea infection rate of rodents were 1.39 ± 0.34 and (35.90 ± 5.34)％, respectively. Xenopsylla Cheopis index was 1.20 and free flea index was 0.009. Flea infection rate was (32.36 ± 0.96)％ between 2005 and 2009, which was lower than (39.44 ± 0.39)％ between 2000 and 2004(t =2.76, P ＜ 0.05). ConclusionsThe major rodent species found in Quanzhou city is Rattus norvegicus. Xenopsylla Cheopis is the dominant flea species.Spreading of plague among Rattus is not found.
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate salvage surgical approaches and efficacy for post-radiation local recurrent nasopharyngeal carcinoma (NPC).</p><p><b>METHODS</b>Thirteen patients with post-radiation local recurrent NPC underwent salvage surgical treatment by routes as transpalatal approach, nasal medial swing approach, maxillary swing approach and infratemporal fossa approach. All cases were followed up for 2 to 5 years. Analysis was done on the indications and efficacy of these 4 different approaching routes.</p><p><b>RESULTS</b>No immediate operative complications occurred for all these 13 cases. Four patients with T1 and T2a operated via transpalatal approach and nasal medial swing approach survived more than 3 years. Five patients with T2b and T3 operated via maxillary swing approach. Among them, two patients died at second and 24th month after operation, one survived with tumor and died at 13rd month after operation, two were alive free of tumor for 2 and 4 years after operation. Four patients with T4 operated via infratemporal fossa approach. Among them, three died in 1 year, one was alive free of tumor for 2 years.</p><p><b>CONCLUSIONS</b>Surgical approaches were decided by a comprehensive consideration of recurrent tumor site and invasive range to achieve the best operative site exposure with minimal traumatic damage.</p>